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Vision Core Facilities

The OU Vision Research Facilities have a state-of-the-art Vision Research Core designed to aid investigators in their vision research. These Modules are funded by a Core grant from the National Institutes of Health / National Eye Institute (P30EY027125 PI: RE Anderson) and an unrestricted grant from Research to Prevent Blindness to the Dean McGee Eye Institute.

Cellular Imaging Module

The Cellular Imaging Module is located in the ground level of the research facility at DMEI. The facility was expanded in March 2013 with the addition of the Olympus MVX10, a macroview-fluorescence microscope system, and the Olympus FV1200 confocal system.  This new Olympus Imaging Suite for Macro to Confocal Imaging is the result of a successful submission of a supplement by Dr. Anderson in May 2012.  The new confocal system prepares us for the eventual phase out of the FV500 confocal system when replacement parts become even more scarce and unavailable.  The FV1200 is equipped with all solid state laser diodes which are stable, long lasting, and energy efficient.  The stage is designed to accommodate an environmental chamber for live cell/tissue imaging.  It is also outfitted with the ultra-sensitive GaAsP detectors for low signal imaging.  The MVX10 system is equipped with a fully motorized stage and the CellSens software for creating stack images and large montages using the multiple image alignment function of the software.  Users can create bright-field and fluorescence images of up to 20X objective equivalent resolution images of flat mount tissues such as retinas and corneas. Also housed in this module is a Leica cryostat for sectioning, Nikon E400 and E800 microscopes, and review stations for Fluoview and Metamorph applications.

The Director of this Module is Dr. Michael Elliott and the Systems Manager of this Module is Dr. Lily Wong.

CIC Reservation Calendar (requires login)

Live Animal Imaging Module

Instrumentation and technical assistance provided by the Live Animal Imaging and Functional Analysis Module are used by nearly every vision researcher on the OUHSC campus. This Module is one of the most active and labor-intensive modules, as nearly all animals of all vision researchers (4000-5000 research animals consisting of rodents, rabbits, and chicks) are analyzed by imaging and functional analysis equipment located in this Module. The Modules contain instrumentation similar to that used in the ophthalmology clinic for patient care, so the findings of analyses of vision disorders in animal models can be translated to human disease.

The LAI Module has two locations on the OUHSC campus, one at DMEI and one at the Biomedical Sciences Building across campus. Each location is in close proximity to vision research labs and the housing facilities which contain vision research animals. Each location contains a complement of the following instruments used in analyzing vision in small animal models: Diagnosys Electroretinography Systems, Bioptigen SD Optical Coherence Tomography, Optomotry, Microns III and IV with slit lamp and laser attachments, Zeiss operating microscopes with MindStream imaging, various support and portable vision analysis equipment, and environmental housing, including dark adaptation, dark rearing, light damage housing, and oxygen and humidity chamber housing.

The Director of this Module is Dr. Michelle Callegan and the Systems Manager of this Module is Dr. Feng Li.

LAI Module Website (scheduling requires Google Calendar access)

Genotyping Module

For researchers who use complex genetically modified mouse models, genotyping is the most tedious and time-consuming process and has zero error tolerance. The Genotyping Module provides fast, accurate, and convenient genotyping service to the mouse research community in the OUHSC campus.  This service is based on conventional PCR and gel electrophoresis methods, and we achieve technical efficiency through our streamlined operation and quality control. The OUHSC vision research labs involve a broad range of transgenic and mutant mouse and chick strains, and fast, accurate genotyping is critical to the analysis and interpretation of the data generated. The Genotyping Module is located at DMEI.

The Director of this Module is Dr. Dan Carr and the Systems Manager of this Module is Ms. Fatemeh Shariati.